Description

EXPRESS METHOD FOR SIMULTANEOUS determination of β-lactams, tetracycline, streptomycin and chloramphenicol in milk samples.

Pack of 96

Measurement time – 10 minutes.

Introduced into GOST R and Interstate Standard

The “4sensor” test is an express method for the simultaneous detection of several types of antibiotics (beta-lactams, tetracycline, streptomycin, levomycetin) in natural, powdered and pasteurized milk. The study uses two components:

1. Microwell
containing a predetermined amount of receptors and antibodies associated with particles of colloidal gold;

2. Test strip
consisting of a set of membranes with fixation lines. The “control” line is printed in green and is permanently visible. Four other lines are special “measuring” lines located below the “control” line. When the reagent from the microwell is mixed with the milk sample, the four receptors bind the corresponding analyte antibiotics, if any, during the first 5 minutes of incubation at 40+3°C. Then, after being immersed in milk, the liquid begins to migrate along the test strip and through the measuring lines. If the sample does not contain antibiotics, the lines will turn deep red. Conversely, the presence of antibiotics in the sample will not lead to color staining of the measurement lines.

Antibiotics
«4Sensor» Units
/g (μg/g)
Penicillin-G 0,002
Ampicillin 0,003
Amoxicillin 0,003
Cloxacillin 0,006
Dicloxacillin 0,006
Oxacillin 0,012
Nafcillin 0.030
Cefacetrile 0.030
Cephapyrin 0,006
Cephalonium 0,003
Cefazolin 0,018
Cefoperazone 0,003
Cefkin 0,020
Ceftiofur 0,010
Chlortetracycline 0,006
Doxycycline 0,0025
Oxytetracycline 0,007
Tetracycline 0,01
Levomycetin 0,003
Streptomycin 0,15
Dihydrostreptomycin 0,05